Milla Jovovich

Alice

Oded Fehr

Carlos Olivera

Ali Larter

Claire

Iain Glen

Dr. Isaacs

Ashanti

Betty

Christopher Egan

Mikey

Spencer Locke

K-Mart

Matthew Marsden

Slater

Linden Ashby

Chase

Jason O'Mara

Albert Wesker

Mike Epps

L.J.

Joe Hursley

Otto

John Eric Bentley

Umbrella Tech

James Tumminia

Lab Tech

Kirk B.R. Woller

Scientist

Rick Cramer

Ice Hockey / Corridor Guard

Madeline Carroll

White Queen

Peter O'Meara

British Envoy

William Abadie

French Envoy

Ramón Franco

Runty

Shane Woodson

Piggy

Valorie Hubbard

Ma

Geoff Meed

Pock Mark

Rusty Joiner

Eddie

Brian Steele

Rancid / Tyrant

Connor McCoy

Small Boy

Gary A. Hecker

Tyrant Vocal (voice)

Richard Epcar

Additional Voices (voice) (uncredited)

Gary Hudson

Umbrella Captain (uncredited)

Carsten Nørgaard

Zombie Steve (uncredited)

César Ámigo Aguilar

Foreign Envoy (uncredited)