Shameik Moore

Miles Morales (voice)

Jake Johnson

Peter B. Parker (voice)

Hailee Steinfeld

Gwen Stacy (voice)

Mahershala Ali

Uncle Aaron (voice)

Brian Tyree Henry

Jefferson Davis (voice)

Lily Tomlin

Aunt May (voice)

Luna Lauren Vélez

Rio Morales (voice)

Zoë Kravitz

Mary Jane (voice)

John Mulaney

Spider-Ham (voice)

Kimiko Glenn

Peni Parker (voice)

Nicolas Cage

Spider-Man Noir (voice)

Kathryn Hahn

Doc Ock (voice)

Liev Schreiber

Wilson Fisk (voice)

Chris Pine

Peter Parker (voice)

Natalie Morales

Miss Calleros (voice)

Edwin H. Bravo

Brooklyn Visions Security Guard (voice)

Oscar Isaac

Interesting Person #1 (voice)

Greta Lee

Interesting Person #2 (voice)

Stan Lee

Stan (voice)

Jorma Taccone

Green Goblin / Last Dude (voice)

Joaquín Cosío

Scorpion (voice)

Marvin Jones III

Tombstone (voice)

Kim Yarbrough

Scientist in Cafeteria (voice)

Lake Bell

Vanessa Fisk (voice)

Jessica Mikayla Adams

Brooklyn Friend (voice)

Gredel Berrios Calladine

Brooklyn Friend (voice)

Sarah D. Cole

Brooklyn Friend (voice)

Kelby Joseph

Brooklyn Friend (voice)

Mimi Davila

Brooklyn Visions Student (voice)

Claudia Choi

Brooklyn Visions Teacher (voice)

Melanie Haynes

Brooklyn Visions Teacher (voice)

Joseph Izzo

Brooklyn Visions Teacher (voice)

Nick Jaine

Brooklyn Visions Teacher (voice)

Muneeb Rehman

Brooklyn Visions Teacher (voice)

Carlos Zaragoza

Brooklyn Visions Teacher (voice)

Post Malone

Brooklyn Bystander (voice)

David Applebee

Additional Voices (voice)

Juan Carlos Arvelo

Additional Voices (voice)

Adam Brown

Additional Voices (voice)

Jon Bruno

Additional Voices (voice)

Darcy Rose Byrnes

Additional Voices (voice)

Oscar Camacho

Additional Voices (voice)

June Christopher

Additional Voices (voice)

Alycia Cooper

Additional Voices (voice)

Michelle Jubilee Gonzalez

Additional Voices (voice)

Terrence Hardy Jr.

Additional Voices (voice)

Bridget Hoffman

Additional Voices (voice)

Rif Hutton

Additional Voices (voice)

Miguel Jiron

Additional Voices (voice)

Harrison Knight

Additional Voices (voice)

Lex Lang

Additional Voices (voice)

Donna Lynn Leavy

Additional Voices (voice)

Andrew Leviton

Additional Voices (voice)

Caitlin McKenna

Additional Voices (voice)

Scott Menville

Additional Voices (voice)

Christopher Miller

Additional Voices (voice)

Arthur Ortiz

Additional Voices (voice)

Juan Pacheco

Additional Voices (voice)

Devika Parikh

Additional Voices (voice)

Shakira Ja'nai Paye

Additional Voices (voice)

Courtney Peldon

Additional Voices (voice)

Chrystee Pharris

Additional Voices (voice)

Jacqueline Pinol

Additional Voices (voice)

Juan Pope

Additional Voices (voice)

Al Rodrigo

Additional Voices (voice)

Joseph Sanfelippo

Additional Voices (voice)

Justin Shenkarow

Additional Voices (voice)

Dennis Singletary

Additional Voices (voice)

Warren Sroka

Additional Voices (voice)

Melissa Sturm

Additional Voices (voice)

Holly Walker

Additional Voices (voice)

Jason Linere-White

Additional Voices (voice)

Ruth Zalduondo

Additional Voices (voice)